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| |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
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| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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| <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
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| ==objective==
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| The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals
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| ==Preparation of stock solutions==
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| # BSA
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| #L-Cysteine
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| #Sodium Hydroxide
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| #Sodium Sulfide
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| ==Protocol BSA:L-cysteine Nanocrystals==
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| # In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate
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| # Add 200 uL of 40 mM Ammonium Acetate
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| # Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein
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| # Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL
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| # Incubate at room temp for 1 hour
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| # Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing
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| # Incubate for five days at 37 degrees Celsius
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| ==Table of BSA:L-Cysteine ratios==
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| {| {{table}}
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| | mol of protein||2.00E-09||1.80E-09||1.60E-09||1.40E-09||1.20E-09||1.00E-09||8.00E-10||6.00E-10||4.00E-10||2.00E-10||0
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| | percentage of protein||100||90||80||70||60||50||40||30||20||10||0
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| | percentage of ligand||0||10||20||30||40||50||60||70||80||90||100
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| | mol of ligand||0||2.00E-10||4.00E-10||6.00E-10||8.00E-10||1.00E-09||1.20E-09||1.40E-09||1.60E-09||1.80E-09||2.00E-09
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| |}
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| |}
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| __NOTOC__
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