User:Andrew K Farag/Notebook/Andrewfarag/2014/01/30

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
 
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==objective==
 
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The objective for today is to begin the synthesis of BSA:L-Cysteine Nanocrystals
 
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==Preparation of stock solutions==
 
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# BSA
 
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#L-Cysteine
 
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#Sodium Hydroxide
 
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#Sodium Sulfide
 
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==Protocol BSA:L-cysteine Nanocrystals==
 
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# In test tube mix 180 uL of 5mM Zinc Nitrate and 20 uL of 5mM Manganese Acetate
 
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# Add 200 uL of 40 mM Ammonium Acetate
 
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# Add 200 uL of Protein:ligand in various ratios from 0% to 100% protein
 
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# Adjust pH to 8.2 using roughly 250 uL of 10mM Ammonium Hydroxide, and bring volume of water to 2.3 mL 
 
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# Incubate at room temp for 1 hour
 
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# Add 200 uL of 5mM Sodium Sulfide drop wise while vortexing
 
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# Incubate for five days at 37 degrees Celsius
 
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==Table of BSA:L-Cysteine ratios==
 
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{| {{table}}
 
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| mol of protein||2.00E-09||1.80E-09||1.60E-09||1.40E-09||1.20E-09||1.00E-09||8.00E-10||6.00E-10||4.00E-10||2.00E-10||0
 
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| percentage of protein||100||90||80||70||60||50||40||30||20||10||0
 
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| percentage of ligand||0||10||20||30||40||50||60||70||80||90||100
 
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| mol of ligand||0||2.00E-10||4.00E-10||6.00E-10||8.00E-10||1.00E-09||1.20E-09||1.40E-09||1.60E-09||1.80E-09||2.00E-09
 
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__NOTOC__
 

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