User:Andrew K Farag/Notebook/CHEM-490/2014/09/08: Difference between revisions

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==Description==
==Description==
I started working this semester, Fall 2014, under the supervision of [[User:Matt Hartings|Dr. Hartings]], with [[User:James C. Schwabacher|James]] and [[User:Eleni N. Kalivas|Eleni]] on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to [http://www.ncbi.nlm.nih.gov/pubmed/22826036|Protein-directed synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing.].
I started working this semester, Fall 2014, under the supervision of [[User:Matt Hartings|Dr. Hartings]], with [[User:James C. Schwabacher|James]] and [[User:Eleni N. Kalivas|Eleni]] on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to [http://www.ncbi.nlm.nih.gov/pubmed/22826036|Protein-directed synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing.].
==Procedure==
*5mL of 5mM HgNO3--HgNO3 was not fully disolving so HgCl2 was used instead
*5mL BSA (15mg/mL)
*final pH around 9 (adjusted by 1M NaOH)--The pH was around 10 in this reaction. Not enough calculations were taken when adding the NaOH.
*The variac was set on 55/100, temperature is around 115C
*vigorous stirring
*stir for 8-12 hours, till color is pale yellow
*Eleni followed the same procedure, however her solution was left in room temperature
*The nitrogen gas and the water supply under the hood was all set for the reflux reaction.
==Calculations==
*HgCl2 in 50 mL of water
*271.5(g/mol)*0.005(mol/L)*0.05L=0.067875g
*BSA in 50 mL of water
*50mL*15(mg/mL)*1(g/1000mg)=0.75g
*NaOH
*40(g/mol)*1(mol/L)*0.05L=2g of NaOH pellets.
--1.9905 g were used

Revision as of 15:42, 16 September 2014

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Description

I started working this semester, Fall 2014, under the supervision of Dr. Hartings, with James and Eleni on Quantum Dots again. The project was started last semester in Chemistry-571, however not enough results were collected. This semester we will be working on synthesizing QDs again using Mercury instead. The procedure is according to synthesis of NIR-emitting, tunable HgS quantum dots and their applications in metal-ion sensing..

Procedure

  • 5mL of 5mM HgNO3--HgNO3 was not fully disolving so HgCl2 was used instead
  • 5mL BSA (15mg/mL)
  • final pH around 9 (adjusted by 1M NaOH)--The pH was around 10 in this reaction. Not enough calculations were taken when adding the NaOH.
  • The variac was set on 55/100, temperature is around 115C
  • vigorous stirring
  • stir for 8-12 hours, till color is pale yellow
  • Eleni followed the same procedure, however her solution was left in room temperature
  • The nitrogen gas and the water supply under the hood was all set for the reflux reaction.

Calculations

  • HgCl2 in 50 mL of water
  • 271.5(g/mol)*0.005(mol/L)*0.05L=0.067875g
  • BSA in 50 mL of water
  • 50mL*15(mg/mL)*1(g/1000mg)=0.75g
  • NaOH
  • 40(g/mol)*1(mol/L)*0.05L=2g of NaOH pellets.

--1.9905 g were used

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