User:Andrew K Farag/Notebook/CHEM-571/2014/02/04: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(3 intermediate revisions by the same user not shown)
Line 25: Line 25:


Note: Much of the protocol for today will be determined by the results of our first synthesis.
Note: Much of the protocol for today will be determined by the results of our first synthesis.
==Edited Protocol==
* we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.
Corrected Volumes Table
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''41.65 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''11uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM L-Cysteine (uL)'''
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0||540||60||600||0||600||3000||600||5.4
|-
| 10||540||60||600||60||540||3000||600||5.4
|-
| 20||540||60||600||120||480||3000||600||5.4
|-
| 30||540||60||600||180||420||3000||600||5.4
|-
| 40||540||60||600||240||360||3000||600||5.4
|-
| 50||540||60||600||300||300||3000||600||5.4
|-
| 60||540||60||600||360||240||3000||600||5.4
|-
| 70||540||60||600||420||180||3000||600||5.4
|-
| 80||540||60||600||480||120||3000||600||5.4
|-
| 90||540||60||600||540||60||3000||600||5.4
|-
| 100||540||60||600||600||0||3000||600||5.4
|}


==Data==
==Data==
* Add data and results here...
All solutions in the test tube have orange fluorescence properties. All test tubes have precipitates, the largest with 0% BSA.
UV-Vis and fluorescence measurements of all test tubes are recorded.
<br>UV-Vis of BSA-L-Cysteine
[[Image:Bsa-cys.png]]


==Notes==
==Notes==

Revision as of 18:55, 25 February 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Objective

Characterize the previously synthesized BSA-L-cysteine ZnS:Mn Nanocrystals using UV-Vis.

Protocol

  1. Remove 1 mL aliquot from test tube and place in the cuvette.
  2. Take the UV-Vis spectra of the sample.
  3. Repeat for each test tube
  4. Take the UV-Vis spectra of each standard: BSA, L-cysteine, zinc (II) nitrate and manganese (II) acetate.
  5. Data analysis of the UV-Vis absorbances
  6. Determine if any changes in procedure need to be made.
    1. Is a different scale of protein:ligand ratio required?
    2. Were QD NCs successfully synthesized?
  7. Prepare stock solutions for next synthesis on the following day.

Note: Much of the protocol for today will be determined by the results of our first synthesis.

Edited Protocol

  • we are going to increase the final volume of solution to yield more mass to be collected. The original final volume will be multiplied by three.


Corrected Volumes Table

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 41.65 mM Ammonium Acetate (uL) 11uM BSA* (uL) 10uM L-Cysteine (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
0 540 60 600 0 600 3000 600 5.4
10 540 60 600 60 540 3000 600 5.4
20 540 60 600 120 480 3000 600 5.4
30 540 60 600 180 420 3000 600 5.4
40 540 60 600 240 360 3000 600 5.4
50 540 60 600 300 300 3000 600 5.4
60 540 60 600 360 240 3000 600 5.4
70 540 60 600 420 180 3000 600 5.4
80 540 60 600 480 120 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

Data

All solutions in the test tube have orange fluorescence properties. All test tubes have precipitates, the largest with 0% BSA. UV-Vis and fluorescence measurements of all test tubes are recorded.
UV-Vis of BSA-L-Cysteine

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Andrew_K_Farag/Notebook/CHEM-571/2014/02/04&oldid=776925"