User:Andrew K Farag/Notebook/CHEM-571/2014/02/05: Difference between revisions

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#0.00278 g in 50mL --> Stock
#0.00278 g in 50mL --> Stock
#569.13uL Stock in 10mL --> 10 uM GSH
#569.13uL Stock in 10mL --> 10 uM GSH
Preparation of BSA-GSH nanocrystal solutions
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''% BSA'''
| align="center" style="background:#f0f0f0;"|'''5 mM Zinc Nitrate (uL)'''
| align="center" style="background:#f0f0f0;"|'''5mM Manganese Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''41.65 mM Ammonium Acetate (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM BSA* (uL)'''
| align="center" style="background:#f0f0f0;"|'''10uM GSH (uL)'''
| align="center" style="background:#f0f0f0;"|'''10mM Ammonium Hydroxide (uL)'''
| align="center" style="background:#f0f0f0;"|'''5 mM Sodium Sulfide* (uL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0||540||60||600||0||600||3000||600||5.4
|-
| 10||540||60||600||60||540||3000||600||5.4
|-
| 20||540||60||600||120||480||3000||600||5.4
|-
| 30||540||60||600||180||420||3000||600||5.4
|-
| 40||540||60||600||240||360||3000||600||5.4
|-
| 50||540||60||600||300||300||3000||600||5.4
|-
| 60||540||60||600||360||240||3000||600||5.4
|-
| 70||540||60||600||420||180||3000||600||5.4
|-
| 80||540||60||600||480||120||3000||600||5.4
|-
| 90||540||60||600||540||60||3000||600||5.4
|-
| 100||540||60||600||600||0||3000||600||5.4
|}


==Protocol for New synthesis method==
==Protocol for New synthesis method==

Revision as of 11:29, 12 February 2014

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  1. Synthesize new nano crystals following the protocol used on Jan 01, 2104
  2. Synthesize new nano crystals using new ligand GSH
  3. Attempt new synthesis method described below


Prepare 10 uM of GSH

  1. 0.00278 g in 50mL --> Stock
  2. 569.13uL Stock in 10mL --> 10 uM GSH


Preparation of BSA-GSH nanocrystal solutions

% BSA 5 mM Zinc Nitrate (uL) 5mM Manganese Acetate (uL) 41.65 mM Ammonium Acetate (uL) 10uM BSA* (uL) 10uM GSH (uL) 10mM Ammonium Hydroxide (uL) 5 mM Sodium Sulfide* (uL) Total Volume (mL)
0 540 60 600 0 600 3000 600 5.4
10 540 60 600 60 540 3000 600 5.4
20 540 60 600 120 480 3000 600 5.4
30 540 60 600 180 420 3000 600 5.4
40 540 60 600 240 360 3000 600 5.4
50 540 60 600 300 300 3000 600 5.4
60 540 60 600 360 240 3000 600 5.4
70 540 60 600 420 180 3000 600 5.4
80 540 60 600 480 120 3000 600 5.4
90 540 60 600 540 60 3000 600 5.4
100 540 60 600 600 0 3000 600 5.4

Protocol for New synthesis method

This protocol was taken from Matt Harting's notebook

  1. To a 3-necked flask is added
    1. 25mL of 20mM L-cysteine
    2. 25mL of 20mM BSA
    3. 5mL of 100mM ZnSO4 (0.5 mmol)
    4. 5.437mL of 10.3mM MnCl2 (0.056 mmol)
    5. The pH of this mixture is adjusted to 11 with ~1M NaOH (should be on the order of 60uL)
  2. This mixture is stirred under Ar (I'll use N2) for 30 minutes
  3. 5.505mL of 101mM Na<usb>2S is quickly added.
  4. The solution is stirred for 20 minutes
  5. The solution is then aged at 50C under air for 2 hours.

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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