User:Andrew K Farag/Notebook/CHEM-581/2014/08/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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## Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring | ## Place film in 1% HCl Solution (see below for details) at 80C for 30 minutes with gentle stirring | ||
## Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring | ## Place film in Sodium Bicarbonate Solution for 15 minutes at room temperature with gentle stirring | ||
##store in water | |||
# Starting new film synthesis | # Starting new film synthesis | ||
## Use [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/08/27|Dr. Hartings' Wednesday's Procedure]] to make new 10% clay-polyvinyl alcohol film | ## Use [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2014/08/27|Dr. Hartings' Wednesday's Procedure]] to make new 10% clay-polyvinyl alcohol film | ||
### It was 10% by weight clay because we used 1g of PVA and 0.11g of clay | ### It was 10% by weight clay because we used 1g of PVA and 0.11g of clay | ||
*PVA weight-1.0029g (Polymer only) | |||
*PVA weight-1.0038g (Polymer and clay) | |||
*Na montmorillonite-0.1105g | |||
# Malachite Green Solutions | # Malachite Green Solutions | ||
## We need to make the following solutions | ## We need to make the following solutions | ||
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## Take a UV-Visible spectrum of the 0.2ppm to 8ppm standards. | ## Take a UV-Visible spectrum of the 0.2ppm to 8ppm standards. | ||
## Make a standard curve to determine the molar absorptivity of Malachite Green | ## Make a standard curve to determine the molar absorptivity of Malachite Green | ||
#Two sets are to be prepared; each set includes a different kind of film (Polymer and Polymer+Clay). The weights of the films should be recorded as well. | |||
[[Image:Films.in.vials.png]] | |||
==Data== | ==Data== | ||
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## This solution will be used to neutralize the acid in your films | ## This solution will be used to neutralize the acid in your films | ||
== | ==The Making of Malachite Green Stock Solutions== | ||
We prepared the 0.20 ppm and 0.50 ppm Malachite Green Stock Solutions. | |||
Melvin, Becky, and Eleni prepared the 1.1 ppm and 1.4 ppm Malachite Green Stock Solutions. | |||
Madeleine and Alicia prepared the 1.7 ppm and 2.0 ppm Malachite Green Stock Solutions. See [[User:Madeleine_Y._Bee/Notebook/CHEM-581_Experimental_Chemistry/2014/08/29|Madeleine's 8/29 Page]] for their calculations. | |||
James, Jake, and Mary prepared the 8.0 ppm, 80.0 ppm, and 200.0 ppm Malachite Green Stock Solutions. See [[User:James_C._Schwabacher/Notebook/CHEM-571/2014/08/29|James's 8/29 Page]] for their calculations. | |||
### 25mL 0.20ppm Malachite Green - made with 0.025mL of 200.0ppm solution in 24.975mL water | |||
### 25mL 0.50ppm Malachite Green - made with 0.0625mL of 200.0ppm solution in 24.9375mL water | |||
### 25mL 1.1ppm Malachite Green - made with | |||
### 25mL 1.4ppm Malachite Green - made with | |||
### 25mL 1.7ppm Malachite Green - made with 5.31mL of 8.0ppm solution in 19.69mL water | |||
### 100mL 2.0ppm Malachite Green - made with 1mL of 200.0ppm solution in 99.0mL water | |||
### 50mL 8.0ppm Malachite Green - made with 2mL of 200.0 ppm solution in 48.0mL water | |||
### 50mL 80.0ppm Malachite Green - made with 20mL of 200.0ppm solution in 30.0 mL water | |||
### 50mL 200.0ppm Malachite Green - made with 20.00mg of MG in 100mL volumetric flask. | |||
==Calculations== | |||
Our calculations for the dilution of the stock solution to solutions 25 mL of 0.20 ppm and 0.50 ppm are as follows: | |||
'''Conc<sub>1</sub>*V<sub>1</sub> = Conc<sub>2</sub>*V<sub>2</sub>''' | |||
*.20ppm | |||
**(.20ppm)(25mL)=(200.0ppm)*V | |||
**V=0.025mL of 200.0ppm solution in 24.975mL water | |||
*0.5ppm | |||
**(0.5ppm)(25mL)=(200ppm)*V | |||
**V=0.0625mL of 200ppm solution in 24.9375mL water | |||
[[Category:Course]] | [[Category:Course]] |
Latest revision as of 00:13, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveCompleting film synthesis, starting new film synthesis, and making stock Malachite green solutions. Description
DataStock Solutions
The Making of Malachite Green Stock SolutionsWe prepared the 0.20 ppm and 0.50 ppm Malachite Green Stock Solutions. Melvin, Becky, and Eleni prepared the 1.1 ppm and 1.4 ppm Malachite Green Stock Solutions. Madeleine and Alicia prepared the 1.7 ppm and 2.0 ppm Malachite Green Stock Solutions. See Madeleine's 8/29 Page for their calculations. James, Jake, and Mary prepared the 8.0 ppm, 80.0 ppm, and 200.0 ppm Malachite Green Stock Solutions. See James's 8/29 Page for their calculations.
CalculationsOur calculations for the dilution of the stock solution to solutions 25 mL of 0.20 ppm and 0.50 ppm are as follows: Conc1*V1 = Conc2*V2
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