User:Andrew K Farag/Notebook/HAT-8/28-AuNP: Difference between revisions
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# Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | # Sodium Citrate (Na<sub>3</sub>C<sub>6</sub>H<sub>5</sub>O<sub>7</sub>·2H<sub>2</sub>O) 0.1010g in 10.0mL → 1.01% | ||
From the | <br>[[Image:Absorbtionvswavelength.png|right|This is the graph of the absorption of the particle vs wave length]] | ||
From the Absorption vs Wave Length Graph, the peak is at a wave length of 518 and absorbance is 0.612. As the peak is at a wave length less than 520, table S-1 from the reference is used. | |||
<br>A<sub>450</sub>=0.389 | <br>A<sub>450</sub>=0.389 | ||
<br>A<sub>518</sub>=0.612 | <br>A<sub>518</sub>=0.612 | ||
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<br>c=0.389*1cm/(1.09*10^8/M/cm) | <br>c=0.389*1cm/(1.09*10^8/M/cm) | ||
<br>c=3.5688*10^-9 M | <br>c=3.5688*10^-9 M | ||
[[Category:Course]] | [[Category:Course]] |
Revision as of 10:52, 3 September 2013
Biomaterials Design Lab | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
ObjectiveSynthesize two different sets of gold nanoparticles. In one set, Au3+ is reduced by a protein (bovine serum albumin, BSA) and the synthesized nanoparticle is also surrounded and stabilized by BSA. In the second set, Au3+ is reduced by citrate, and the AuNP is stabilized by citrate in solution. The BSA-AuNPs are purple in color and the citrate-AuNPs are more of a burgundy (reddish) color. BSA-AuNPStart by placing all of your materials into a volumetric flask so that you know the exact volumes and exact amounts of what you have added. I will have prepared the initial protein and gold solutions for you (this one time only). In the future, you will be expected to make most of the starting solutions. This procedure was taken from the following reference and has been used by our previous two Experimental Biological Chemistry groups.
Stock solutions made
citrate-AuNPThis procedure is used by Dr. Miller in her research lab - Allison Alix's notebook and is taken from this reference and can be analyzed according to this reference.
Stock solutions made
From the Absorption vs Wave Length Graph, the peak is at a wave length of 518 and absorbance is 0.612. As the peak is at a wave length less than 520, table S-1 from the reference is used.
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