User:Andrew W Long/Notebook/Protein Modification/2014/09/22: Difference between revisions
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== | ==Procedure for Reactions Done with the Ocean Optics Instrument== | ||
Today we continued the same type of experiments that we have done for the past few months. The procedure is as follows: | Today we continued the same type of experiments that we have done for the past few months. The procedure is as follows: | ||
'''Lab bench setup:''' | |||
*Fill an ~8 Qt container with ice, then add water until mostly full | |||
*Place pump portion of the Ocean Optics & the end of the yellow tube in the ice bath | |||
**Note that there are two sections of yellow tubing. One section should be connected to the pump and the Ocean Optics machine. The other should have one end connected to the Ocean Optics machine, while the other is allowed to rest/drain back into the ice bath. | |||
'''Computer and Instrument setup:''' | |||
*Turn on the Jaz apparatus of the Ocean Optics instrument (simply press the power button, no other buttons need to be pressed) | |||
*Turn on the light source apparatus of the Ocean Optics instrument (switch on the back of the machine), then turn on the Deuterium lamp (blue button). Allow the Deuterium lamp to be on for 30 minutes (or until finished flashing) before you begin the experiment, and before you turn on the Halogen lamp. | |||
*Turn on the Halogen lamp. | |||
*Open the Q-Blue Wireless Temperature Control software. Check the box for control status (it should read "on"), change the target temperature to 25 degrees C, and make sure the stirrer is on and set to 1000 rpm. | |||
'''When making the solutions for the Ocean Optics:''' | '''When making the solutions for the Ocean Optics:''' | ||
Revision as of 10:05, 22 September 2014
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Procedure for Reactions Done with the Ocean Optics InstrumentToday we continued the same type of experiments that we have done for the past few months. The procedure is as follows: Lab bench setup:
Computer and Instrument setup:
When making the solutions for the Ocean Optics:
When putting solutions into cuvette:
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