User:Andrew W Long/Notebook/Protein Modification/2014/11/21: Difference between revisions

Latest revision as of 00:32, 27 September 2017

Project name

Today, 20 ml of KCl, Mb, and Phosphate buffer solution was prepared

We used 0.0025 mol of acid component (monobasic phosphate) and 0.0024 mol basic component (dibasic phosphate) and 500 ml of water, then used 20 ml of that
- Calculated using www.biomol.net/en/tools/buffercalculator.htm
We added 5 mg of Mb and 500 mg of KCl
We then flash froze the solution in liquid nitrogen, and lyophilized it

Isopropanol

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