User:Andrew W Long/Notebook/Protein Modification/2014/11/21: Difference between revisions
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==Preparing KCl, Mb, in pH7 Phosphate buffer solution== | ==Preparing KCl, Mb, in pH7 Phosphate buffer solution AND New Ocean Optics Procedure== | ||
Today, 20 ml of KCl, Mb, and Phosphate buffer solution was prepared | Today, 20 ml of KCl, Mb, and Phosphate buffer solution was prepared | ||
*We used 0.0025 mol of acid component (monobasic phosphate) and 0.0024 mol basic component (dibasic phosphate) and 500 ml of water, then used 20 ml of that | *We used 0.0025 mol of acid component (monobasic phosphate) and 0.0024 mol basic component (dibasic phosphate) and 500 ml of water, then used 20 ml of that | ||
*We added 5 mg of Mb and 500 mg of KCl | *We added 5 mg of Mb and 500 mg of KCl | ||
*We then flash froze the solution in liquid nitrogen, and lyophilized it | *We then flash froze the solution in liquid nitrogen, and lyophilized it | ||
Isopropanol | |||
*Scan every 10 seconds for 10 minutes 1ms integration time, average 1 scan | |||
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Revision as of 14:06, 21 November 2014
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Preparing KCl, Mb, in pH7 Phosphate buffer solution AND New Ocean Optics ProcedureToday, 20 ml of KCl, Mb, and Phosphate buffer solution was prepared
Isopropanol
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