User:Andy Maloney/Kinesin & Microtubule Page/K-PEM

Recipe

• PEM
• 1.0 mg/mL kappa casein

Procedure

See the [math]\displaystyle{ \alpha }[/math]-PEM procedure for pictures.

1. Weigh out 25 mg of kappa casein.
2. Add 25 mL of PEM to the casein.
3. Place in a beaker with a stir bar and stir for approximately 15-20 minutes on setting 7, or until all the casein is in solution.
4. Aliquot into vials and store in the 4°C fridge.

Do not filter solution. This casein solution will last for 6 months in the fridge.