User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29/Lipid stuff

Solubility

I am actually quite confused as to why my lipids didn't go completely into my 1:1 MeOH:EtOH solution. I'm told by the technicians from Avanti that I can sonicate my sauce to see if the lipids go into solution as well as heating them up. I do not want to use the hot plate to heat up the lipids because too much evaporation will occur. I will use the PCR machine once Ant is done checking it out for calibration purposes.

Drying chamber

I also pretty sure that I need to dry out the solvents better. Unfortunately the pump we have is not designed to pump continuously when already at a "vacuum". So, I made a little "drying chamber" that allows me to pump in a low flow of nitrogen and at the same time pull a vacuum on it.

I would also like to add that the above image was my first "note" taken with Evernote. Yes, they do kick major awesome ass!

I think my favorite is the drying rack, which is nothing more than a pencil eraser with cut outs.

Heavy PEM

Alas, I have done everything today without realizing that I have to sit and wait for things. I swapped out the hard drives in Kiney's computer and I am reinstalling the operating system which, will take hours. So, I will just have to wait to start this.