User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/10/29/Lipid stuff: Difference between revisions
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==Heavy PEM== | ==Heavy PEM== | ||
Alas, I have done everything today without realizing that I have to sit and wait for things. I swapped out the hard drives in Kiney's computer and I am reinstalling the operating system which, will take hours. So, I will just have to wait to start this. | Alas, I have done everything today without realizing that I have to sit and wait for things. I swapped out the hard drives in Kiney's computer and I am reinstalling the operating system which, will take hours. So, I will just have to wait to start this. | ||
[[User:Steven J. Koch|Steve Koch]] 00:31, 30 October 2009 (EDT): I'll show you how to do the backup script using SyncToy2.0 soon. It's a decent backup strategy. Dealing with movies is still a major issue that I don't know how to deal with. Even though our server is 2 TB, it'll fill up. I'm inclined to just keep buying more RAID servers, since archiving to tape or whatever is almost as inconvenient as deleting them. Maybe the UNM library can help? I have in mind that they only deal with putting published stuff in the repository (as opposed to ALL data) ... but maybe I'm wrong. |
Revision as of 21:31, 29 October 2009
Solubility
I am actually quite confused as to why my lipids didn't go completely into my 1:1 MeOH:EtOH solution. I'm told by the technicians from Avanti that I can sonicate my sauce to see if the lipids go into solution as well as heating them up. I do not want to use the hot plate to heat up the lipids because too much evaporation will occur. I will use the PCR machine once Ant is done checking it out for calibration purposes.
Drying chamber
I also pretty sure that I need to dry out the solvents better. Unfortunately the pump we have is not designed to pump continuously when already at a "vacuum". So, I made a little "drying chamber" that allows me to pump in a low flow of nitrogen and at the same time pull a vacuum on it.
I would also like to add that the above image was my first "note" taken with Evernote. Yes, they do kick major awesome ass!
I think my favorite is the drying rack, which is nothing more than a pencil eraser with cut outs.
Heavy PEM
Alas, I have done everything today without realizing that I have to sit and wait for things. I swapped out the hard drives in Kiney's computer and I am reinstalling the operating system which, will take hours. So, I will just have to wait to start this. Steve Koch 00:31, 30 October 2009 (EDT): I'll show you how to do the backup script using SyncToy2.0 soon. It's a decent backup strategy. Dealing with movies is still a major issue that I don't know how to deal with. Even though our server is 2 TB, it'll fill up. I'm inclined to just keep buying more RAID servers, since archiving to tape or whatever is almost as inconvenient as deleting them. Maybe the UNM library can help? I have in mind that they only deal with putting published stuff in the repository (as opposed to ALL data) ... but maybe I'm wrong.