User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2009/11/25/Sucrose: Difference between revisions
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==Notes== | |||
These guys definitely look like they are running slower than the motility assays I have done without osmolytes in them. Of course, I cannot determine how much slower until I analyze the movies made. | |||
Revision as of 15:22, 25 November 2009
Setup
- I polymerized microtubules.
- I put 2 M sucrose in PEM.
- I prepared from the 2 M sucrose solution, 3 dilutions of 0.25 M, 0.50 M, and 0.75 M sucrose in the motility solutions.
- I made my flow cells.
Motility
So I did change some things up from my heavy water experiments. Instead of doing the sucrose dilutions sequentially in time, I prepared all of them at the same time. I did however, not flow in the microtubules until after I took data for one slide.
I have made movies that are 2 minutes long for one field of view. The playback rate is 5 frames/s and the capture rate was 5 Hz. The images have an exposure of 100 ms and a gain of 150.
250 mM Sucrose
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500 mM Sucrose
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750 mM Sucrose
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Notes
These guys definitely look like they are running slower than the motility assays I have done without osmolytes in them. Of course, I cannot determine how much slower until I analyze the movies made.
