User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/04/01/Osmotic stress planning

Planning

The first chemical I am going to use as an osmotic stessor will be betaine. I want to complete 3 initial points to the data taking. 2 extreme points and one point between. I already have my 0 mM betaine point so I will take data for the following:

• 1000 mM betaine
• 5 mM betaine
• 60 mM betaine

I understand that the 60 mM point isn't really between 1000 and 5 but, I am going to approach 1000 mM logarithmically. So if I approximately double the concentration from 5 to 1000, 60 is the middle point for me and what I plan to do.

Software needed

Since I've adopted the term software to mean chemicals, biological materials, and solutions, I need to prepare a 2M solution of betaine in PEM. There are some things to take into consideration when preparing this solution.

• I don't want the betaine to directly interact with my casein. This means that no betaine will go into my casein stocks.
• Due to my preliminary data on caseins, I will use alpha casein for my surface passivator.
• Betaine will only be added to motility solutions. This means I only need 2M betaine in PEM. I will dub this solution PEM-Bine. (Ultimately I will look at sarcosine and proline and those solutions will be called PEM-Sine and PEM-Pine respectively.)