User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/20: Difference between revisions

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==Entry title==
{{AndyMaloneyNotebook
* Insert content here...
|Description=Making new solutions.
}}
[[Category:Kinesin and microtubules]]
[[Category:AM_Engineering]]
[[Category:AM Surface passivation paper]]
==New Sauce==
So I went ahead and prepared more PEM. However, I did change the following:
 
* Prepared a 10x PEM solution.
* Filtered through a 200nm filter.
* Aliquoted into 1mL tubes and put them in the -80C freezer. I'm placing a 6 month shelf life on the PEM so in 6 months, all aliquots will need to be discarded and a fresh solution will need to be made.
 
I also received my new stock caseins. This means I am going to prepare new solutions containing them. Again, it will be 0.5mg/mL solutions of the various caseins. Filtered and aliquoted and placed in the -80C freezer.




Revision as of 20:15, 23 October 2010

Lab notebook of Andy Maloney

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New Sauce

So I went ahead and prepared more PEM. However, I did change the following:

  • Prepared a 10x PEM solution.
  • Filtered through a 200nm filter.
  • Aliquoted into 1mL tubes and put them in the -80C freezer. I'm placing a 6 month shelf life on the PEM so in 6 months, all aliquots will need to be discarded and a fresh solution will need to be made.

I also received my new stock caseins. This means I am going to prepare new solutions containing them. Again, it will be 0.5mg/mL solutions of the various caseins. Filtered and aliquoted and placed in the -80C freezer.



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