User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/29/Beta casein test

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Yesterday, I found out that none of my casein solutions were supporting motility except for my whole casein. I have narrowed the reasons for this down to the possibility that it was storing them in the -80C freezer. Today, I prepared more beta-PEM and I'm going to try an assay to see if it works. I did send the solution through a 0.2um filter.

First try

Nope...No motility on the slip. I did see a small amount on the slide but I can't use that. I'll wait a few more minutes to see if anything is working.

15 minutes in and still nothing.


I don't get it. It just isn't working. And of course I didn't save some beta-PEM to not filter it so I could see if that was the issue.

  • The Taxol is working just fine because the MTs are stable.
  • The Antifade is working because after blasting them with 100% illumination, they are still bright.
  • The kinesin is good as has been shown with the whole casein assay. As well as the PEM-Glu and PEM-A are good because whole casein works.
  • The freezer isn't the problem as was shown right now.
  • The filtering can't be the problem because the whole casein works. Unless the filter is gobbling up all the casein. But then I wouldn't see bubbles forming in my casein solutions, which I do so there is protein in them.

This truly has become a mystery now. Maybe if I get a new batch of kinesin I'll try this again. But, I'm out of ideas and there is no point trying to brute force anything now.


After 1.5 hours, nothing.

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