User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/08/12/Casein study: Difference between revisions
Andy Maloney (talk | contribs) (New page: {{AndyMaloneyNotebook |Description=Continuing the data taking for my passivation study. I've noticed that kappa and beta casein don't work very well when incubated at room temp. So, I'm g...) |
Andy Maloney (talk | contribs) |
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==Mixed casein== | ==Mixed casein== | ||
Since the mixed casein is basically whole casein and I know already that it will work, I'm going to try it with the new aliquot of kinesin I got. If I still see the same sort or loose ends, then I'm going to try a different motility solution with a different aliquot of antifade. | Since the mixed casein is basically whole casein and I know already that it will work, I'm going to try it with the new aliquot of kinesin I got. If I still see the same sort or loose ends, then I'm going to try a different motility solution with a different aliquot of antifade. | ||
It appears that the kinesin was the issue. I have a motility assay working right now and it looks spectacular with the mixed caseins. | |||
Revision as of 12:57, 12 August 2010
Whole casein
I want to make sure that everything is working, so I'm going to start off with my trusty whole casein experiment.
- Movie 1
- Seems to be not a lot of motility going on. That will change though.
- Movie 2
- I'm getting a lot of guys that have their minus ends in solution.
It really looks like my kinesin may have gone bad. I think this may be the case because I'm getting a lot of loose ends and sticking going on. Of course, it could be what I found out yesterday which was that BME is necessary for motility to work. If this is the case, then I'm screwed.
I'm going to go ahead and take an entire run just to see if the assay is robust enough to give similar speed data. I am however going to get another aliquot of kinesin and try again with a passivation that I know will work.
Mixed casein
Since the mixed casein is basically whole casein and I know already that it will work, I'm going to try it with the new aliquot of kinesin I got. If I still see the same sort or loose ends, then I'm going to try a different motility solution with a different aliquot of antifade.
It appears that the kinesin was the issue. I have a motility assay working right now and it looks spectacular with the mixed caseins.
