User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study: Difference between revisions
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So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not. | So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not. | ||
Even the 6% Hg illumination is working. It's weird what happened yesterday. | |||
==26% O-PEM trial 01== | |||
No problems with things disintegrating at the beginning of an assay. It definitely was the antifade since that was the only thing that I changed from yesterday to today. Besides the microtubules. | |||
Revision as of 10:08, 12 October 2010
Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of.
40% H-PEM trial 01
Just to change things up from yesterday, I'm going to do a D2O study first.
So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not.
Even the 6% Hg illumination is working. It's weird what happened yesterday.
26% O-PEM trial 01
No problems with things disintegrating at the beginning of an assay. It definitely was the antifade since that was the only thing that I changed from yesterday to today. Besides the microtubules.
