User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study: Difference between revisions
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[[Category:Kinesin and microtubules]] | [[Category:Kinesin and microtubules]] | ||
[[Category:AM_Water isotope study]] | [[Category:AM_Water isotope study]] | ||
[[Category:AM_Engineering]] | |||
Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of. | Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of. | ||
==40% H-PEM trial 01== | ==40% H-PEM trial 01== | ||
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==63% O-PEM trial 01== | ==63% O-PEM trial 01== | ||
Things are working great. Time to take the data. | Things are working great. Time to take the data. | ||
==The engineering portion of the day== | |||
I had to make new flow cells today as well as new antifade. I also removed the micrometers from the microscope stage because they were starting to irritate me. Low an behold I remembered why I installed them in the first place, the Olympus stage irritates me even more than the micrometers do. Suffice it to say, I need to come up with a better solution than the one I have and just using the stock Olympus crap. |
Revision as of 11:21, 12 October 2010
Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of.
40% H-PEM trial 01
Just to change things up from yesterday, I'm going to do a D2O study first.
So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not.
Even the 6% Hg illumination is working. It's weird what happened yesterday.
26% O-PEM trial 01
No problems with things disintegrating at the beginning of an assay. It definitely was the antifade since that was the only thing that I changed from yesterday to today. Besides the microtubules.
63% O-PEM trial 01
Things are working great. Time to take the data.
The engineering portion of the day
I had to make new flow cells today as well as new antifade. I also removed the micrometers from the microscope stage because they were starting to irritate me. Low an behold I remembered why I installed them in the first place, the Olympus stage irritates me even more than the micrometers do. Suffice it to say, I need to come up with a better solution than the one I have and just using the stock Olympus crap.