User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study

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Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of.

40% H-PEM trial 01

Just to change things up from yesterday, I'm going to do a D2O study first.

So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not.