User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/10/12/Water isotope study
Since I had problems of microtubules falling apart at the beginning of experiments yesterday, I decided to make new antifade. Hopefully this is the reason as to why things were not working yesterday and I can document it as such. If not, then there is something else going on that I am not aware of.
40% H-PEM trial 01
Just to change things up from yesterday, I'm going to do a D2O study first.
So I believe it was the antifade that was causing the initial problems with the assay as I have not had any problem with this one. Although, there seem to be a bit less MTs in solution than there usually are. Not sure if this is just an artifact of me being able to observe the slide quickly or not.
Even the 6% Hg illumination is working. It's weird what happened yesterday.
26% O-PEM trial 01
No problems with things disintegrating at the beginning of an assay. It definitely was the antifade since that was the only thing that I changed from yesterday to today. Besides the microtubules.
