User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/24/Reusable flow cell
Reusable flow cell
After letting the reusable flow cell sit on the counter of the weekend, there has been minimal evaporation. I am very impressed with this new setup and I look forward to using it in experiments.
New water isotope study
The new study will try to look at the old data I took but to do it in one flow cell. There are problems with this as I have no clue as if or when the flow cell passivation will undergo a breakdown. In other words, passivation will fail and all the kinesin and passivation will flow ou t of the chamber.
As a first test to this new setup would be to make a flow cell like I normally would and then "seal" it with the static vinyl. Take data as I normally would and see if the data matches what I would get with the nail polish.
Once I have determined that this new setup is equivalent to the old setup, I can try multiple fluid exchanges with one flow cell. Unfortunately, I won't be able to get to this experiment today as there is a ton of setup for it.
Preparations
To prepare for this experiment, I have to:
- Change the bulb in the mercury lamp.
- Make new antifade.
- Order new passivation chemicals.
- Order new antifade chemicals.
- Make new flow cells.
- Re-epoxy thermistor to the objective.
