User:Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2011/01/25/Reusable flow cell: Difference between revisions
Andy Maloney (talk | contribs) (New page: {{AndyMaloneyNotebook |Description=Testing the reusable flow cell. }} Category:Kinesin and microtubules Category:AM_Engineering Category:AM_Water isotope study ==Initial trial...) |
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[[Category:AM_Water isotope study]] | [[Category:AM_Water isotope study]] | ||
==Initial trial== | ==Initial trial== | ||
This trial will consist of me using: | |||
* The 100W Hg lamp. | |||
* 1.0 mg/mL alpha casein passivation. 10 minute incubation. | |||
* Fluid exchange with 0.5 mg/mL alpha casein with 27μg/mL kinesin and 1 mM ATP. 5 minute incubation using twice as much fluid to passivate. | |||
* Fluid exchange with regular motility solution using 20 μL. | |||
I will take data for about 20 minutes or 10 data points. This allows me to get the slide to the objective temperature with enough stable data points to be able to determine the average speed. Once I have taken this data, the slide will come off of the microscope and a fluid exchange with the same regular motility solution will occur. Again, 20 μL of fluid and 10 data points. I am going to repeat this for a total of 9 times or until I see something catastrophic occur in the data taking. | |||
The 9 times is because there will be 9 different solutions of fluid exchange for the water isotope study. Hopefully my fluid exchanges will not affect the passivation and kinesin adhesion on the slide. But, this is what needs to be determined. | |||
Revision as of 10:18, 25 January 2011
Initial trial
This trial will consist of me using:
- The 100W Hg lamp.
- 1.0 mg/mL alpha casein passivation. 10 minute incubation.
- Fluid exchange with 0.5 mg/mL alpha casein with 27μg/mL kinesin and 1 mM ATP. 5 minute incubation using twice as much fluid to passivate.
- Fluid exchange with regular motility solution using 20 μL.
I will take data for about 20 minutes or 10 data points. This allows me to get the slide to the objective temperature with enough stable data points to be able to determine the average speed. Once I have taken this data, the slide will come off of the microscope and a fluid exchange with the same regular motility solution will occur. Again, 20 μL of fluid and 10 data points. I am going to repeat this for a total of 9 times or until I see something catastrophic occur in the data taking.
The 9 times is because there will be 9 different solutions of fluid exchange for the water isotope study. Hopefully my fluid exchanges will not affect the passivation and kinesin adhesion on the slide. But, this is what needs to be determined.
