User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/20: Difference between revisions
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==Results== | ==Results== | ||
[[Image:PH 5 fluor 160920 fluorescence.PNG]] | |||
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[[Image:Fluor ph5 bsa part 2 160920.PNG]] | [[Image:Fluor ph5 bsa part 2 160920.PNG]] | ||
The chart above shows that the integrated fluorescence increases rapidly from ~4000-7500 nm in the first 20 min. The integrated fluorescence then continues to increase more slowly from ~7500-8500 for the next 20 min before reaching an asymptote around ~9000 nm for the rest of the experiment. | The chart above shows that the integrated fluorescence increases rapidly from ~4000-7500 nm in the first 20 min. The integrated fluorescence then continues to increase more slowly from ~7500-8500 for the next 20 min before reaching an asymptote around ~9000 nm for the rest of the experiment. | ||
==UV-Vis Solutions for tomorrow== | ==UV-Vis Solutions for tomorrow== |
Revision as of 12:35, 28 September 2016
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Solution PreparationBSA Fluorescence Solution
Stock Concentrations 160920
Volumes for making the solution:
MethodsThe sample was put in the fluorimeter and measurements were taken every five minutes for three hours. ResultsThe chart above shows the emission maximums over time for the solution of pH 5 BSA with gold nanoparticles. A major increase in maximum emission occurs around ~7 minutes and another smaller increase occurs around ~17 minutes. The emission maximum then plateaus around 420-440 nm. The chart above shows that the integrated fluorescence increases rapidly from ~4000-7500 nm in the first 20 min. The integrated fluorescence then continues to increase more slowly from ~7500-8500 for the next 20 min before reaching an asymptote around ~9000 nm for the rest of the experiment. UV-Vis Solutions for tomorrowStock Solutions:
Solutions were put in the oven at 80'C and held for four hours.
Fluorescence vs. Ocean Optics pH 5
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