User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/27: Difference between revisions

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[[Image:OO pH6 part1 160928.PNG]]
[[Image:OO pH6 part1 160928.PNG]]
The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s.
[[Image:OO pH6 part3 160928.PNG]]
The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run.


[[Image:OO pH6 part2 160928.PNG]]
[[Image:OO pH6 part2 160928.PNG]]


[[Image:OO pH6 part3 160928.PNG]]
The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.
 


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Revision as of 11:32, 28 September 2016

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BSA Solution

  1. BSA Ocean Optics Solution
    1. pH 6
    2. [Au]=0.25 mM
    3. [BSA]=3.125 uM
    4. Vf=3000 uL
  2. Stock Concentrations 160927
    1. [BSA]= 52.6 uM
    2. [Au]= 4.27 mM
    3. [HCl]= 1mM
  3. Volumes for making the solution:
    1. 3 uL HCl
    2. 294.6 uL BSA
    3. 292.7 uL Au
    4. 2409.5 uL HOH

Results

The chart above shows a dramatic increase in maximum wavelength from about ~450 to ~550 at about ~3200s.

The chart above shows a slight decrease in max absorbance until about 1200 sec. at which point it dramatically increases for the rest of the run.

The chart above shows a slight decrease in A(535) until about 1200 sec. at which point it dramatically increases for the rest of the run. This is similar to the maximum absorbance over time chart as expected.



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