User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/18: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Sample Prep== | |||
BSA Ocean Optics Solution | |||
# pH 8 | |||
# [Au]=0.25 mM | |||
# [BSA]=3.125 uM | |||
# Vf=3000 uL | |||
Stock Concentrations 160920 | |||
# [BSA]= 63.1 uM | |||
# [Au]= 2.39 mM | |||
# [NaOH]= 1 mM | |||
Volumes for making the solution: | |||
# 148.9 uL BSA | |||
# 255.6 uL Au | |||
# 2565.4 uL HOH | |||
# 3 uL NaOH | |||
==Results and Data Analysis== | |||
[[Image:OO 18 meme part 1.PNG]] | |||
The above chart displays the absorbance at 535.15 nm of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart shows a gradual increase in absorbance over the run time. | |||
[[Image:OO 18 meme part 2.PNG]] | |||
The above chart shows the maximum wavelength (nm) of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart displays a plateau of max wavelength ~485 nm until about ~3800 seconds, where it jumps dramatically to about ~550 nm; then leveling off between 530 and 540 nm. | |||
[[Image:OO 18 meme part 3.PNG]] | |||
The above chart shows the gradual increase in the maximum absorbance of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. | |||
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Latest revision as of 02:07, 27 September 2017
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Entry title
Sample PrepBSA Ocean Optics Solution
Stock Concentrations 160920
Volumes for making the solution:
Results and Data AnalysisThe above chart displays the absorbance at 535.15 nm of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart shows a gradual increase in absorbance over the run time. The above chart shows the maximum wavelength (nm) of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart displays a plateau of max wavelength ~485 nm until about ~3800 seconds, where it jumps dramatically to about ~550 nm; then leveling off between 530 and 540 nm. The above chart shows the gradual increase in the maximum absorbance of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. |