User:Anthony Lazzaro / SANDBOX

Pictures:

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Download Link :

MyPresentation

Anchoring

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Citations for PubMed

[1, 2]

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Links

Internal : internal

External : ign

Penny Arcade

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Time Stamp

Anthony Lazzaro 11:23, 12 July 2007 (EDT)

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Google specific search

Search Term site :openwetware.org

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Well Characterised Part in Registry:

Recievers:

BBa_F2620 :

• Input: 3OC6HCL
• Output: Pops

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Summary of Brainstorming 18th July:

Hrp has promise as a black box feedback loop mechanism

• Application Thermoregulation
• General application to a system that needs feedback loop - duh!

Hrp has promise as a very specific switch

Other Promising Ideas:

Lucas: Bacteria that move away from a source

• Could expand to bacteria that move away or towards a chemical source & incorporate feedback loop seen in signals
  • Possible conflict with chemotaxis (?) from last year need to check could reuse there parts if no conflict

Density flow receptor: Not only detect presence of bacteria but also how much of it there is a particular location
eg.how much dust here compared to here

• Need some kind of measurement device or quorum sensing set up (so more stuff more cells more reporter)

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Uses for Hrp System: (Blood testing)

Immediate blood-test results can mean the difference between life and death in medical situations such as poisonings and infectious diseases. In many cases, by the time the test results are analyzed and a diagnosis made, the patient is in critical condition or beyond treatment

link

Blood poisoning is an illness due to an infectious agent or its toxin spreading through the bloodstream. The presence of bacteria in the blood is called bacteremia.

Short bursts of low levels of bacteria in the blood usually do not cause problems. For example, mild bacteremia typically occurs during a dental cleaning or when brushing your teeth. Your body's immune system fights off these bacteria.

If bacteria persist in the blood, however, they may cause sepsis, a serious, life-threatening condition.

link

• Slovenia has already covered this what other blood test could we do ?

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20th July

Further Brainstorming for new ideas:

All my ideas have been rejected so far

Avenues to explore for new ideas:

1.Pre-2006 iGEMs

• 2005:

2.Registry 3.New Parts in Papers (start with reviews)

Focus on Adhesion:

1.Easy Application

-Make Bacteria Bind to something when told to do so

• E. coli MC1061 synthesize type I pili encoded by the fim operon, which bind to

mammalian surface carbohydrates [3]

• We could use this to simply bind bacteria to carbohydrates when the operon is expressed

(eg. simple promoter activation)

4. Anderson JC, Clarke EJ, Arkin AP, and Voigt CA. Environmentally controlled invasion of cancer cells by engineered bacteria. J Mol Biol. 2006 Jan 27;355(4):619-27. DOI:10.1016/j.jmb.2005.10.076 |

*Focus on Biofilm:

1.Easy Application

Secrete Biofilm when inducer given:

Quick note on Noise reduction in iGEM

I think one way to reduce noise & cross talk in iGEM projects is to have 'comm channels'

'Channels' used by the chassis should be chara