User:Anthony Salvagno/Notebook/Research/2009/05/04/Hairpin Digestion

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Revision as of 09:58, 4 May 2009 by Anthony Salvagno (talk | contribs) (New page: {{#widget:Google Spreadsheet |key=rnpoCHkyhhm5nfprB22fo0Q|width=800|height=200}} The hairpin oligo has a NotI site in it and so we are doing 2 digestions. One with the enzyme and one wi...)
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{{#widget:Google Spreadsheet |key=rnpoCHkyhhm5nfprB22fo0Q|width=800|height=200}}

The hairpin oligo has a NotI site in it and so we are doing 2 digestions. One with the enzyme and one without. The reasoning for the second digestion is so that we can make sure there isn't anything weird going on while the oligo is in the 37C chamber.