User:Anthony Salvagno/Notebook/Research/2010/10/20/Tethering Doing: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (Quick-adding category Ant Tethering (using HotCat)) |
|||
Line 5: | Line 5: | ||
*Also the DNA I had was 11pM which is kinda sad, but for some reason I think it came out that way after PCR. I did find another batch that was 22nM so I will dilute that 100x to get me some 220pM stuff. | *Also the DNA I had was 11pM which is kinda sad, but for some reason I think it came out that way after PCR. I did find another batch that was 22nM so I will dilute that 100x to get me some 220pM stuff. | ||
*Next I will tether with small and big beads. | *Next I will tether with small and big beads. | ||
[[Category:Ant Tethering]] | [[Category:Ant Tethering]] | ||
==Tethering Experiment== | |||
I made two aliquots of 4.4kb DNA. I'm going to try to tether with the 3 different concentrations I have: 2nM, 200pM, and 11pM. | |||
{{ShowGoogleExcel|id=0Agbdciapt4QZdHBWXzZoeXF2ZUdweHhPNV9UNWJvaEE|width=500|height=300}} |
Latest revision as of 09:30, 20 October 2010
Quick Navigation: Pages by Category | List of All Pages | Protocols
So yesterday I got some good feedback from Koch.
Done So Far
- I talked to Andy and he said BGB should be good for about 6 months in a salt solution. I told him my situation (dissolved in POP) and he thought it should be good. Since I made it June 29, I deem it alright. For now I will try it out. But I will make a new batch pretty soon.
- I've diluted more anti-dig in PBS.
- Also the DNA I had was 11pM which is kinda sad, but for some reason I think it came out that way after PCR. I did find another batch that was 22nM so I will dilute that 100x to get me some 220pM stuff.
- Next I will tether with small and big beads.
Tethering Experiment
I made two aliquots of 4.4kb DNA. I'm going to try to tether with the 3 different concentrations I have: 2nM, 200pM, and 11pM. {{#widget:Google Spreadsheet |key=0Agbdciapt4QZdHBWXzZoeXF2ZUdweHhPNV9UNWJvaEE |width=500 |height=300 }}