User:Anthony Salvagno/Notebook/Research/2011/02/14/Finishing Unzipping Construct:Ligation Attempts: Difference between revisions
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==Results from Last Week== | ==Results from Last Week== | ||
Well I had a misstep in the lab. While I was purifying the Adapter-DpBR ligation (aka ApBR) I felt a splash as if I got some of it on my face. Now after purification I did get some result so the only way to know what I got is to do the final ligation and gel analysis. While this is going I will run the other ligation simultaneously. | Well I had a misstep in the lab. While I was purifying the Adapter-DpBR ligation (aka ApBR) I felt a splash as if I got some of it on my face. Now after purification I did get some result so the only way to know what I got is to do the final ligation and gel analysis. While this is going I will run the other ligation simultaneously. | ||
Anyways the nanodrop says I have 40nM of ApBR. We'll see. | |||
==Unzipping Ligation== | ==Unzipping Ligation== | ||
{{ShowGoogleExcel|id=0Agbdciapt4QZdExRV3M2RzJyUmktc2djcndTSEtkOGc|width=500|height=300}} | {{ShowGoogleExcel|id=0Agbdciapt4QZdExRV3M2RzJyUmktc2djcndTSEtkOGc|width=500|height=300}} | ||
Revision as of 10:18, 14 February 2011
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Results from Last Week
Well I had a misstep in the lab. While I was purifying the Adapter-DpBR ligation (aka ApBR) I felt a splash as if I got some of it on my face. Now after purification I did get some result so the only way to know what I got is to do the final ligation and gel analysis. While this is going I will run the other ligation simultaneously.
Anyways the nanodrop says I have 40nM of ApBR. We'll see.
Unzipping Ligation
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Adapter-DpBR Ligation 2
{{#widget:Google Spreadsheet |key=0Agbdciapt4QZdGJoTi1GVGVBWXpvWVpmMFlFQjRFbkE |width=500 |height=300 }} Same ligation as Friday, and I'm using the same recipe so I don't feel the need to do anything differently notebook wise.
