User:Anthony Salvagno/Notebook/Research/2011/04/13/pALS PCR Troubleshooting
From OpenWetWare
(Difference between revisions)
(New page: {{qn}} ==Previous== The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear...) |
(→PCR Setup) |
||
| Line 4: | Line 4: | ||
==PCR Setup== | ==PCR Setup== | ||
| + | {{ShowGoogleExcel|id=0Agbdciapt4QZdGdTbW1ORy1GeGpJMGhnWHR0NFNDUWc|width=500|height=300}} | ||
Revision as of 14:01, 13 April 2011
Quick Navigation: Pages by Category | List of All Pages | Protocols
Previous
The temperature in the lab has forced me to lower the temperature on the thermal cycler to 50C for annealing and that produced a PCR product. It also gave me less smear than I typically receive when doing this experiment so that is positive. I will now try to do PCR with labeled reverse primer and unlabeled forward primer and work my way through the tethering construct construction.
PCR Setup
| View/Edit Spreadsheet |
