User:Anugraha Raman/Notebook/iGEM 2010/2010/06/21: Difference between revisions
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* Lab Presentation | * Lab Presentation | ||
* Primer Design | * Primer Design | ||
** | ** (Amplify 300 bp exon following our intron in Fra a 1) | ||
***Took first/last 20 sequences of exon | |||
** (Amplify out intron for use (but this contains biobrick cloning sites so we probably won't do this) | |||
***Took last 5 sequences in exon, first 15 sequences in intron and last 15 sequences in intron and first 5 of following exon) | |||
* GFP Sequence Alignment | * GFP Sequence Alignment | ||
[[Image:GFPalignment.png|thumb|left|too many mismatches in alignment]] | [[Image:GFPalignment.png|thumb|left|too many mismatches in alignment]] | ||
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* PCR (Bet v1, Bet v 1.2, Ger 3, LTP 1) | * PCR (Bet v1, Bet v 1.2, Ger 3, LTP 1) | ||
** Set annealing temp for 67.5 | |||
** Used ~ 100 ng of genomic aribidopsis DNA/ 50 uL reaction | |||
Revision as of 14:11, 21 June 2010
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