User:Anugraha Raman/Notebook/iGEM 2010/2010/06/25: Difference between revisions
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==Friday June 25, 2010== | ==Friday June 25, 2010== | ||
* '''Digestions''' w/ purified DNA from yesterday. | * '''Digestions''' w/ purified DNA from yesterday. | ||
**LTP S: 20.4 ng/uL | **LTP S: 20.4 ng/uL | ||
**LTP A: 12.2 ng/uL | **LTP A: 12.2 ng/uL | ||
**Bet1 S: 15.4 ng/uL | **Bet1 S: 15.4 ng/uL | ||
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**Total: 40 uL Volume | **Total: 40 uL Volume | ||
**Normal Digest (Digested for 1 hour) | **Normal Digest (Digested for 1 hour) | ||
*Concentrations after purification | |||
**LTP S: 36.3 ng/uL | |||
**LTP A: 32.1 ng/uL | |||
**Bet1 S: 30 ng/uL | |||
**Bet1 A: 34 ng/uL | |||
**Bet 1.2 S: 32.7 ng/uL | |||
**Bet 1.2 A: 34.3 ng/uL | |||
**Ger 3 S: 11.2 ng/uL | |||
**Ger 3 A: 33.3 ng/uL | |||
*'''PCR amiRNA''' | *'''PCR amiRNA''' | ||
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**Reaction 3: Fwd Primer "primer 1" Rev Primer (B) for LTP/GFP/Betv1 | **Reaction 3: Fwd Primer "primer 1" Rev Primer (B) for LTP/GFP/Betv1 | ||
**Annealing Temp for the three GFP reactions was 67 C and annealing temp for rest of reactions was 60 | **Annealing Temp for the three GFP reactions was 67 C and annealing temp for rest of reactions was 60 | ||
*Concentrations after purification | |||
**GFP 1; 9.1 ng/uL | |||
**GFP 2: 6.2 ng/uL | |||
**GFP 3: 18.3 ng/uL | |||
**LTP1: 9.9 ng/uL | |||
**LTP2: 7.3 ng/uL | |||
**LTP3: 16.2 ng/uL | |||
**Bet1: 2.6 ng/uL | |||
**Bet2: .5 ng/uL | |||
**Bet3: 3 ng/uL | |||
Revision as of 14:24, 25 June 2010
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Friday June 25, 2010
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