User:Aram Kang/Notebook/Analysis of biofilm gene expression/2008/12/26: Difference between revisions
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==Preparation of electrocompetent cell and electroporation == | ==Preparation of electrocompetent cell and electroporation == | ||
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* GYT preparation(250ml) | * GYT medium preparation(250ml) | ||
** 25ml glycerol (10% v/v) | ** 25ml glycerol (10% v/v) | ||
** 0.31g yeast extract (0.125% w/v) | ** 0.31g yeast extract (0.125% w/v) | ||
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**pour 200ul sample each into a agar plate, spread with L shape spreader. | **pour 200ul sample each into a agar plate, spread with L shape spreader. | ||
**place in room temperature to make agar absorb liquid, and then incubate at 37°C (8:30pm) | **place in room temperature to make agar absorb liquid, and then incubate at 37°C (8:30pm) | ||
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* '''LB + kan agar plate preparation''' | |||
** LB broth 12.5g | |||
** agar 10g | |||
** kanamycin 10mg/ml stock solution 2.5ml(working conc. 50μg/mL) | |||
Revision as of 07:10, 26 December 2008
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Preparation of electrocompetent cell and electroporation
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