User:Aram Kang/Notebook/Analysis of biofilm gene expression/2008/12/26: Difference between revisions
From OpenWetWare
Line 19: | Line 19: | ||
** 10:10am--> Measure OD: 37°C -> 0.165 x3=0.495/30°C-> 0.137x3=0.411 | ** 10:10am--> Measure OD: 37°C -> 0.165 x3=0.495/30°C-> 0.137x3=0.411 | ||
** 10:25am--> Measure OD: 37°C -> 0.194 x3=0.582 --> incubate in ice water bath | ** 10:25am--> Measure OD: 37°C -> 0.194 x3=0.582 --> incubate in ice water bath | ||
** 10:45am--> Measure OD: 30°C -> 0.208 x3=0. --> incubate in ice water bath | ** 10:45am--> Measure OD: 30°C -> 0.208 x3=0.624 --> incubate in ice water bath | ||
** centrifuge 25ml aliquots of sample(8 tubes) at 1000g, 4°C, 15min, then discard supernatant carefully and resuspend the cell pellet in 25ml of ice cold sterilized milliQ water.(volume ratio 1:1=sample aliquot:water) | ** centrifuge 25ml aliquots of sample(8 tubes) at 1000g, 4°C, 15min, then discard supernatant carefully and resuspend the cell pellet in 25ml of ice cold sterilized milliQ water.(volume ratio 1:1=sample aliquot:water) | ||
** centrifuge 25ml aliquots of sample(8 tubes) at 1000g, 4°C, 20min, then discard supernatant carefully and resuspend the cell pellet in 12.5ml of ice cold sterilized milliQ water. combine two aliquot 12.5ml into 25ml(volume ratio, 2:1=sample aliquot:water) | ** centrifuge 25ml aliquots of sample(8 tubes) at 1000g, 4°C, 20min, then discard supernatant carefully and resuspend the cell pellet in 12.5ml of ice cold sterilized milliQ water. combine two aliquot 12.5ml into 25ml(volume ratio, 2:1=sample aliquot:water) |
Revision as of 02:22, 29 December 2008
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Preparation of electrocompetent cell and electroporation
|