User:Asya L. Tucker/Notebook/Asya 571/2015/10/07
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ObjectiveMeasure protease Trypsin kinetics with Bradford Assay using 1µM of trypsin. ProcedureIn order to do the Bradford Assay of Protease Degradation, we followed Dr. Hartings' protocol, and did the following specific steps:
Calculations: V1 = [(1µM)(1mL)]/66.09µM = 0.01513mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.01513mL = 0.98487mL DataResults |