User:Asya L. Tucker/Notebook/Asya 571/2015/11/03: Difference between revisions
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Volume of buffer: 1mL - 0.01mL = 0.99mL | Volume of buffer: 1mL - 0.01mL = 0.99mL | ||
==Data== | ==Data== | ||
[[Image:Graph_10_uM_Trypsin.Abs_vs_Wl.png|700px]] | |||
[[Image:Graph_10_uM_Trypsin.Abs_vs_time_I.png|700px]] | |||
[[Image:Graph_10_uM_Trypsin.Abs_vs_time_II.png|700px]] | |||
[[Image:Graph_10_uM_Trypsin.Abs_vs_time_III.png|700px]] | |||
[[Image:Graph_10_uM_Trypsin.Abs_vs_time_IIII.png|700px]] | |||
==Results== | ==Results== | ||
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Revision as of 07:33, 1 December 2015
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ObjectiveMeasure protease Trypsin's kinetics with the Bradford Assay using 10 nM of protease (Trypsin) at different heating time intervals. ProcedureThe general protocol detailed in Dr. Hartings' notebook was used. The following specific steps were performed:
Calculations: 1st Dilution V1 = [(0.01µM)(1mL)]/63.52µM = 0.000157mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.000157mL = 0.9843mL 2nd Dilution V1 = [(1.0µM)(1mL)]/63.52µM = 0.0157mL, amount of trypsin solution needed Volume of buffer: 1mL - 0.01mL = 0.99mL DataResults |