User:Asya L. Tucker/Notebook/Asya 571/2015/11/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
* Insert content here...


==Procedure==
*Ocean Optics
#Centrifuged falcon tubes containing gold fibers.
#Removed water with pipette.
#Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
#The sample must contain:
##Gold fibers
##(2.996mL) of Tris buffer
##(0.003994mL) of trypsin, calculations are below
##The trypsin was added last, at the second reading such that second reading becomes time 0.
#We ran our sample overnight.
#Readings were taken every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.
Calculations for volume of trypsin, and volume of buffer
    C1·V1 = C2·V2,
      where (C1 = 50.21µM)
            C2 = 1µM
            V2 = 1000µL
    (V1 = 0.0199mL)
    C1·V1 = C2·V2,
      where (C1 = 1µM)
            C2 = 0.01µM
            V2 = 3000µL
    (V1 = 30µL)
==Data==
[[Image:RAMOceanoptics_10nM.png|700px]]
[[Image:Graph_OO_10_uM_Trypsin.Abs_vs_time_II.png|700px]]
[[Image:Graph_OO_10_uM_Trypsin.Abs_vs_time_III.png|700px]]
==Results==


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Latest revision as of 01:23, 27 September 2017

Project name Main project page
Previous entry      Next entry

Procedure

  • Ocean Optics
  1. Centrifuged falcon tubes containing gold fibers.
  2. Removed water with pipette.
  3. Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
  4. The sample must contain:
    1. Gold fibers
    2. (2.996mL) of Tris buffer
    3. (0.003994mL) of trypsin, calculations are below
    4. The trypsin was added last, at the second reading such that second reading becomes time 0.
  5. We ran our sample overnight.
  6. Readings were taken every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.

Calculations for volume of trypsin, and volume of buffer 

    C1·V1 = C2·V2,
     where (C1 = 50.21µM)
           C2 = 1µM 
           V2 = 1000µL
    (V1 = 0.0199mL) 

    C1·V1 = C2·V2,
     where (C1 = 1µM)
           C2 = 0.01µM 
           V2 = 3000µL
    (V1 = 30µL)

Data

Results


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