User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/15: Difference between revisions

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==Entry title==
 
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#VM2.3 - BC
#rRNA measurements - FM & BC
#*Overall ratio of O-rRNA/wt-rRNA
#**Harvest
#**RNA extraction
#**Northern blot with probes specific to o/wt rRNA
#*Efficiency of rRNA processing
#**Need to figure out all possible states that the 16s could be in
#**Harvest
#**Native lysis
#**Load onto sucrose gradient
#**spin
#**fractionate
#**Look at A260
#**Combine fractions
#Model - BC




Revision as of 08:55, 15 June 2008

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  1. VM2.3 - BC
  2. rRNA measurements - FM & BC
    • Overall ratio of O-rRNA/wt-rRNA
      • Harvest
      • RNA extraction
      • Northern blot with probes specific to o/wt rRNA
    • Efficiency of rRNA processing
      • Need to figure out all possible states that the 16s could be in
      • Harvest
      • Native lysis
      • Load onto sucrose gradient
      • spin
      • fractionate
      • Look at A260
      • Combine fractions
  3. Model - BC



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