User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/15: Difference between revisions

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#rRNA measurements - FM & BC
#rRNA measurements - FM & BC
#*Figure out more about RnaseI
#*Figure out more about RnaseI
#**[http://www.epibio.com/item.asp?ID=347 Epicenter product page]
#*Overall ratio of O-rRNA/wt-rRNA
#*Overall ratio of O-rRNA/wt-rRNA
#**Harvest
#**Harvest

Revision as of 09:07, 15 June 2008

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  1. VM2.3 - BC
    • Streak tonight
    • Start cultures tomorrow night
    • Measure Tuesday night/Wednesday morning?
    • Reread old protocols
  2. rRNA measurements - FM & BC
    • Figure out more about RnaseI
    • Overall ratio of O-rRNA/wt-rRNA
      • Harvest
      • RNA extraction
      • Northern blot with probes specific to o/wt rRNA
    • Efficiency of rRNA processing
      • Need to figure out all possible states that the 16s could be in
      • Harvest
      • Native lysis
      • Load onto sucrose gradient
      • spin
      • fractionate
      • Look at A260
      • Combine fractions
  3. Model - BC



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