User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/06/20: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 10: Line 10:
*Grow cells
*Grow cells
*Lyse (denaturing lysis is fine)
*Lyse (denaturing lysis is fine)
*Can we store samples
*How do we store lysates
*Design and order probes
*Design and order probes
*Do test Northerns
*Design and order a standard to compare hybridization efficiency.
*Do test Northerns to check primer.
*Decide if we should try to get quantitative measures of rRNA levels or simply pursue measurements relative to wild-type rRNA levels.


==Timeline==
==Timeline==

Revision as of 10:45, 20 June 2008

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Things to do

  • Purchase reagents for RNA extraction via Sean's protocol
  • Purchase reagents for Northern blotting according to Brian's protocol
  • Grow cells
  • Lyse (denaturing lysis is fine)
  • How do we store lysates
  • Design and order probes
  • Design and order a standard to compare hybridization efficiency.
  • Do test Northerns to check primer.
  • Decide if we should try to get quantitative measures of rRNA levels or simply pursue measurements relative to wild-type rRNA levels.

Timeline

  • Order reagents today
  • Do extractions Tuesday/Wednesday
  • Do trial Northern Thursday
  • Try polysome stuff the following week

Questions for Brian

  • Any problems with RNAse when doing the native lysis?
  • Protocol for Northerns
  • Equipment needed to image gel


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Barry_Canton/Notebook/T7_RNAP_transcription_of_rRNA/2008/06/20&oldid=214184"