User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/02: Difference between revisions

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*Order chemifluorescent detection substrate.
*Order chemifluorescent detection substrate.
*Research in vitro transcription to design better standards.
*Research in vitro transcription to design better standards.
==Primer design information==
>wt
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA
>mutant
TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTA
wt16sProbe1
*5'-GGT TGG ATC ACC TCC-3' 49
wt16sProbe2
*5'-GTT GGA TCA CCT CCT TA-3' 48  - ordered this
o16sProbe1
*5'-GTT GGA TCA TTG TGG-3' 44
o16sProbe2
*5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this




Revision as of 15:05, 2 July 2008

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  • Obtain detection substrate from Bryan
  • Order chemifluorescent detection substrate.
  • Research in vitro transcription to design better standards.

Primer design information

>wt TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTA >mutant TGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCATTGTGGTA

wt16sProbe1

  • 5'-GGT TGG ATC ACC TCC-3' 49

wt16sProbe2

  • 5'-GTT GGA TCA CCT CCT TA-3' 48 - ordered this

o16sProbe1

  • 5'-GTT GGA TCA TTG TGG-3' 44

o16sProbe2

  • 5'-GTT GGA TCA TTG TGG TTA-3' 47 - ordered this



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