User:Barry Canton/Notebook/T7 RNAP transcription of rRNA/2008/07/30: Difference between revisions
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*To get this, I added 281μl or 209μl respectively to the primers | *To get this, I added 281μl or 209μl respectively to the primers | ||
Labeled wt probe for about 2.5 hrs and then left on ice for about 2.5 hrs. I took the TOP10 extract and did 1:2 dilutions into DEPC water. I added 5μl of the extraction and 5 dilutions onto fresh membrane. I crosslinked for 5 min at high power on the big Sauer lab UV transilluminator. I prepared fresh hybridization buffer and incubated buffer plus membrane at 62C for a few minutes, I then added all the prepared probe and put at 30C in a roller. Will leave overnight. | |||
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Latest revision as of 13:54, 30 July 2008
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