Tasklist
- Prepare Stock Solutions
- 0.15 M NaOH prepared (3.00g of NaOH in 500 mL with DI water) + (NaOH is hygroscopic and the final concentration will not be able to be measured accurately/ it cannot be used as a primary standard)
- 200 μM isoleucine, aspartic acid, histidine, tyrosine and alanine stock solutions. 100 mL solutions were prepared.
- 200 μmol/L × 131.17 g/mol × 0.1 L = 0.00262 g of isoleucine (calculation for all solutions: 0.00266 g of aspartic acid, 0.00310 g of histidine, 0.00362 g of tyrosine, 0.00178 g of alanine)
- Standardize titrant
- Titrate Amino Acids
- Potassium Hydrogen Phthalate (KHPhth) standard for NaOH. (0.501 g of KHPhth +17 mL of water + titrated with NaOH stock solution + 5 drops of Phenolphthalein/indicator)
- Titration repeated two times + yielded same result
- Final concentration of NaOH = average of results from the three trials = 0.1485 M.
| Trial
|
KHPhth in solution (g)
|
NaOH titrated (mL)
|
| 1 |
0.501 |
16.70
|
| 2 |
0.518 |
17.10
|
| 3 |
0.519 |
17.10
|
- UV/VIS Spectra
- Fluorescence Spectra
- Collect data/sample from DSC
|
Project name
