User:Beatriz Gimenez De C./Notebook/571/2014/09/17

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Task

  • Make dilution of HCL stock solution and Glycine stock solution (made last class)
  • Record conductivity and pH solutions
  • Make a pH and conductivity curve
  • Prepare and run SDS-PAGE Running-Buffer
  • Analyze dialyzed solutions from yesterday

Dilutions

  • Made 8 dilutions in 14 mL Falcon tubes using the volumes of stock solution and water. For each stock solution
  • Diluted ×2, 5, 10, 20, 50, 100, 200, 500.
Dilutions Nacl molarity (mM) pH conductivity (μS/cm)
2 25 6.064 3030
5 10 5.963 1178
10 5 6.189 623
20 2.5 5.989 304
50 1 6.188 290.2
100 0.5 6.507 68
200 0.25 6.331 75.4
500 0.1 6.768 50.8
Glycine dilution Molarity (mM) pH Conductivity (μS/cm)
2 25 3.479 383
5 10 3.6 201.1
10 5 3.704 130.5
20 2.5 3.835 93.9
50 1 4.282 43.8
100 0.5 4.336 35.6
200 0.25 4.762 25.98
500 0.1 5.381 86



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Beatriz_Gimenez_De_C./Notebook/571/2014/09/17&oldid=824873"