User:Beatriz Gimenez De C./Notebook/571/2014/09/24: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/09/24 Entry for User:Beatriz_Gimenez_De_C./Notebook/571)
 
(fix raw html notebook nav)
 
(3 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Tasks==
* Insert content here...
*continue analysis of dialysis data of 9/23/2014
 
** Bradford analysis
[[Image:9-23 dyalysis bard data.png]]
*Prepare 0.5M acetic acid and 0.3M Na2SO4 solution (14.3 mL of acetic acid, 21.3060g were aded to 500 mL volumetric flask and the rest was filled with HPLC water)
* Make dilutions of 1g/L of lysozyme to 0.5 g/L and 0.1 g/L lysozyme.
* Run HPLC with a GPC (Gel Permeation Chromatography) column.
** Ran MEMBRANE FILTRATION of solvent prior to running HPLC,
** Filtered 0.5 M HAc 0.3 M NaSO4 under a vacuum to ensure all particles were <0.45 microns to degas (get rid of CO2 gas)
** Set HPLC machine to: Waters 1525 Binary HPLC pump, flow of A at 0.8 mL/min
** Used syringe filters prior to the injection for lysozyme and standard samples.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 00:21, 27 September 2017

Project name Main project page
Previous entry      Next entry

Tasks

  • continue analysis of dialysis data of 9/23/2014
    • Bradford analysis

  • Prepare 0.5M acetic acid and 0.3M Na2SO4 solution (14.3 mL of acetic acid, 21.3060g were aded to 500 mL volumetric flask and the rest was filled with HPLC water)
  • Make dilutions of 1g/L of lysozyme to 0.5 g/L and 0.1 g/L lysozyme.
  • Run HPLC with a GPC (Gel Permeation Chromatography) column.
    • Ran MEMBRANE FILTRATION of solvent prior to running HPLC,
    • Filtered 0.5 M HAc 0.3 M NaSO4 under a vacuum to ensure all particles were <0.45 microns to degas (get rid of CO2 gas)
    • Set HPLC machine to: Waters 1525 Binary HPLC pump, flow of A at 0.8 mL/min
    • Used syringe filters prior to the injection for lysozyme and standard samples.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Beatriz_Gimenez_De_C./Notebook/571/2014/09/24&oldid=1015473"