User:Beatriz Gimenez De C./Notebook/571/2014/09/24: Difference between revisions
From OpenWetWare
No edit summary |
No edit summary |
||
| Line 8: | Line 8: | ||
==Tasks== | ==Tasks== | ||
*Prepare 0.5M acetic acid and 0.3M Na2SO4 solution (14.3 mL of acetic acid, 21.3060g were aded to 500 mL volumetric flask and the rest was filled with HPLC water) | *Prepare 0.5M acetic acid and 0.3M Na2SO4 solution (14.3 mL of acetic acid, 21.3060g were aded to 500 mL volumetric flask and the rest was filled with HPLC water) | ||
* Make dilutions of 1g/L of | * Make dilutions of 1g/L of lysozyme to 0.5 g/L and 0.1 g/L lysozyme. | ||
* Run HPLC with a GPC (Gel Permeation Chromatography) column | * Run HPLC with a GPC (Gel Permeation Chromatography) column. | ||
** Ran MEMBRANE FILTRATION of solvent prior to running HPLC, | |||
** Filtered 0.5 M HAc 0.3 M NaSO4 under a vacuum to ensure all particles were <0.45 microns to degas (get rid of CO2 gas) | |||
** Set HPLC machine to: Waters 1525 Binary HPLC pump, flow of A at 0.8 mL/min | |||
** Used syringe filters prior to the injection for lysozyme and standard samples. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
Revision as of 11:54, 30 September 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Tasks
| |
