User:Beatriz Gimenez De C./Notebook/571/2014/10/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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* Prepare dialysis of 0.6 g/L Lysozyme vs KI
* Prepare dialysis of 0.6 g/L Lysozyme vs KI  
** Use 3500 MWCO tubing
** Use 3500 MWCO tubing
** Add 1 mL 0.6 g/L Lysozyme solution to 5 cells on one side of chamber
** Add 1 mL 0.6 g/L Lysozyme solution to 5 cells on one side of chamber
** Add 1 mL 2 mM, 5 mM, 10 mM, 25 mM, and 50 mM KI to the opposite side of the chamber (one concentration per cell)
** Add 1 mL 2 mM, 5 mM, 10 mM, 25 mM, and 50 mM KI to the opposite side of the chamber (one concentration per cell)
** Secure wells by screwing them to prevent any evaporation
** Follow protocols previously used
** Place on low speed shaker for one week (Used 0.6 g/ L Lysozyme prepared by Monika)
 
==Note==
 
*Used 0.6 g/ L Lysozyme prepared by Monika




Latest revision as of 00:28, 27 September 2017

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Tasklist

  • Analyze data of dialyzed 30:1 Colloid Solutions against CaCl2 set on October 21.
    • Bradford Analysis
      • 100 μL sample + 200 μL Bradford Reagent diluted (1:3) + 700 μL 50 mM Tris/ 50 mM NaCl buffer.
      • Run UV-Vis between 400 nm and 800 nm
    • UV-Vis analysis
    • Fluorescence analysis.
    • Ca2+ ISE
KI concentration mV measurement (mV)
2mM -198.3
5 mM -217.3
10 mM -237.0
25 mM -247.4
50mM -278.0
  • Prepare dialysis of 0.6 g/L Lysozyme vs KI
    • Use 3500 MWCO tubing
    • Add 1 mL 0.6 g/L Lysozyme solution to 5 cells on one side of chamber
    • Add 1 mL 2 mM, 5 mM, 10 mM, 25 mM, and 50 mM KI to the opposite side of the chamber (one concentration per cell)
    • Follow protocols previously used

Note

  • Used 0.6 g/ L Lysozyme prepared by Monika



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