User:Beatriz Gimenez De C./Notebook/571/2014/10/22: Difference between revisions

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==Tasklist==
==Tasklist==


* Data analysis of dialyzed 30:1 Colloid Solutions against CaCl<sub>2</sub> that were set on October 21.
* Analyze data of dialyzed 30:1 Colloid Solutions against CaCl<sub>2</sub> set on October 21.
** Bradford Analysis of Extracted Colloid Solutions
** Bradford Analysis
*** 100 μL sample + 200 μL Bradford Reagent diluted (1:3) + 700 μL 50 mM Tris/ 50 mM NaCl buffer.
*** 100 μL sample + 200 μL Bradford Reagent diluted (1:3) + 700 μL 50 mM Tris/ 50 mM NaCl buffer.
*** Run UV-Vis between 400 nm and 800 nm  
*** Run UV-Vis between 400 nm and 800 nm  
**UV-Vis and Fluorescence.
** UV-Vis analysis
** Fluorescence analysis.
**Ca<sup>2+</sup> ISE
**Ca<sup>2+</sup> ISE
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Revision as of 18:25, 31 October 2014

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Tasklist

  • Analyze data of dialyzed 30:1 Colloid Solutions against CaCl2 set on October 21.
    • Bradford Analysis
      • 100 μL sample + 200 μL Bradford Reagent diluted (1:3) + 700 μL 50 mM Tris/ 50 mM NaCl buffer.
      • Run UV-Vis between 400 nm and 800 nm
    • UV-Vis analysis
    • Fluorescence analysis.
    • Ca2+ ISE
KI concentration mV measurement (mV)
2mM -198.3
5 mM -217.3
10 mM -237.0
25 mM -247.4
50mM -278.0
  • Prepare dialysis of 0.6 g/L Lysozyme vs KI
    • Use 3500 MWCO tubing
    • Add 1 mL 0.6 g/L Lysozyme solution to 5 cells on one side of chamber
    • Add 1 mL 2 mM, 5 mM, 10 mM, 25 mM, and 50 mM KI to the opposite side of the chamber (one concentration per cell)
    • Secure wells by screwing them to prevent any evaporation
    • Place on low speed shaker for one week (Used 0.6 g/ L Lysozyme prepared by Monika)



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