User:Beatriz Gimenez De C./Notebook/572/2015/01/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Tasks== | ==Tasks== | ||
* Produce the lysozyme AuNP fibers | * Produce the lysozyme AuNP fibers (Made 50, 5 mL samples) | ||
* Weigh 4 mg of Pepsine protease into 8 tubes | * Weigh 4 mg of Pepsine protease into 8 tubes | ||
* Prepare buffer | * Prepare buffer | ||
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* Lysozyme-AuNP fibers preparation | * Lysozyme-AuNP fibers preparation | ||
** Prepare Lysozyme 20 μM stock solution | ** Prepare Lysozyme 20 μM stock solution | ||
*** Measured 0.0261 g of lysozyme into a 100 mL volumetric flask and filled the rest with distilled water | |||
** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ** Prepare 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> stock solution | ||
*** added 0.08539 g of HAuCl4 into a 100 mL volumetric flask and filled the rest with distilled water | |||
** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | ** Transfer 0.5 mL 2.5×10<sup>-3</sup> M HAuCl<sub>4</sub> into 50 culture tubes | ||
*** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for | *** 0.5×10<sup>-3</sup>(L)*2.5×10<sup>-3</sup>(mol/L)= 1.25 (mol) needed for a 45:1 ratio | ||
** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | ** Transfer 1.39 mL of lysozyme stock solution into 50 culture tubes | ||
*** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio | *** 2.77777778×10<sup>-8</sup>(mol)/20*10^-6(mol/ml)= 1.39 mL for a 45:1 ratio | ||
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** 6. 0.00465 g | ** 6. 0.00465 g | ||
** 7. 0.00780 g | ** 7. 0.00780 g | ||
** 8. 0. | ** 8. 0.00584 g | ||
* Prepared 5 mM Tris/0.2 M NaCl buffer for reaction of the synthesized lysozyme-AuNP fibers with the proteases | * Prepared 5 mM Tris/0.2 M NaCl buffer for reaction of the synthesized lysozyme-AuNP fibers with the proteases |
Latest revision as of 00:39, 27 September 2017
Project name | Main project page Next entry |
Tasks
Procedure
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